- Oct 29, 2018
Subject: RNA structure and stress response in plants: from single transcripts to ecological implications
About the Speaker
University: Pennsylvania State University
Abstract: RNA structure is intimately connected to RNA function. Because RNA structure is highly sensitive to the physico-chemical environment, the structure of the same RNA molecule can differ radically in vitro vs. in vivo. We developed a method, Structure-seq, which combines chemical probing of RNA structure with high throughput sequencing to allow characterization of RNA secondary structure in vivo and genome-wide (1-3). We have recently applied Structure-seq to rice seedlings with the goal of revealing general principles underlying RNA structure changes induced by temperature stress. These data will be discussed, along with thoughts concerning the ecological relevance of the RNA structurome.